Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier

• Aniela Bittner,
• Angélique D. Ducray,
• Hans Rudolf Widmer,
• Michael H. Stoffel and
• Meike Mevissen

Beilstein J. Nanotechnol. 2019, 10, 941–954, doi:10.3762/bjnano.10.95

• , namely co-culture or 3D models that mimic the in vivo situation more closely. Experimental Cell culture The immortalized rat brain capillary endothelial cell line rBCEC4 was characterized and kindly provided by Dr. Ingolf E. Blasig (Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany

• times. PCL- and PLLA-NP-stock solutions were then diluted 1:10 in cell culture medium, resulting in concentrations of 2.9 × 1010 PCL-NPs in 1 mL culture medium and 2.6 × 1010 PLLA-NPs in 1 mL culture medium. These concentrations correspond to [24.9 µg/mL]. Au-NPs exhibiting size and surface

• characteristics similar to those of the Si-NPs used were purchased from Nanopartz (Nanopartz Inc., USA). They were 80 nm in diameter, with a zeta potential of −35 mV. Au-NPs were sonicated for 5 min in a sonication bath and vortexed for 2 min prior to dilution in cell culture medium. rBCEC4 cells were exposed to

Polydopamine-coated Au nanorods for targeted fluorescent cell imaging and photothermal therapy

• Boris N. Khlebtsov,
• Andrey M. Burov,
• Timofey E. Pylaev and
• Nikolai G. Khlebtsov

Beilstein J. Nanotechnol. 2019, 10, 794–803, doi:10.3762/bjnano.10.79

• used a Cary Eclipse spectrofluorometer. Cytotoxicity assay The in vitro cytotoxicity was measured using a standard resazurin (Alamar blue) assay following the manufacturer instructions. HeLa cells (1 × 105 cells/well) were seeded into 96-well cell-culture plate and then incubated for 24 h at 37 °C

Surface plasmon resonance enhancement of photoluminescence intensity and bioimaging application of gold nanorod@CdSe/ZnS quantum dots

• Siyi Hu,
• Yu Ren,
• Yue Wang,
• Jinhua Li,
• Junle Qu,
• Liwei Liu,
• Hanbin Ma and
• Yuguo Tang

Beilstein J. Nanotechnol. 2019, 10, 22–31, doi:10.3762/bjnano.10.3

• . Additionally, we investigated the PL signal from a single particle, which indicated a stronger PL intensity compared to that of CdSe/ZnS QDs alone. We also prepared the GNR@CdSe/ZnS modified with folic acid (FA) in cell culture for biological applications. These studies indicated that these multifunctional

Characterization and influence of hydroxyapatite nanopowders on living cells

• Przemyslaw Oberbek,
• Tomasz Bolek,
• Adrian Chlanda,
• Seishiro Hirano,
• Sylwia Kusnieruk,
• Julia Rogowska-Tylman,
• Ganna Nechyporenko,
• Viktor Zinchenko,
• Wojciech Swieszkowski and
• Tomasz Puzyn

Beilstein J. Nanotechnol. 2018, 9, 3079–3094, doi:10.3762/bjnano.9.286

• line (A549), both showing the effect on cells of the respiratory system, which is important for inhalation-exposure assessment during preparation and processing of nanoscale hydroxyapatites. Cell culture All cells were maintained in 35 or 60 mm Φ culture dishes and passaged when 85–90% confluence was

• chromatography, protein purification, cell-culture substrates, catalyst production and waste management. Lower crystallinity, high purity and high zeta potential of hydroxyapatite nanoparticles are desired in a material intended for long-term medical use in the body. Since calcium deficiency is a feature of

The nanoscaled metal-organic framework ICR-2 as a carrier of porphyrins for photodynamic therapy

• Jan Hynek,
• Sebastian Jurík,
• Martina Koncošová,
• Jaroslav Zelenka,
• Ivana Křížová,
• Tomáš Ruml,
• Kaplan Kirakci,
• Ivo Jakubec,
• František Kovanda,
• Kamil Lang and
• Jan Demel

Beilstein J. Nanotechnol. 2018, 9, 2960–2967, doi:10.3762/bjnano.9.275

• ), absolute ethanol (EtOH, Fischer Sci.), FeCl3·6H2O, formamide, and phosphate-buffered saline suitable for cell culture (all Sigma-Aldrich) were used as purchased. Phenylene-1,4-bis(methylphosphinic acid) was prepared according to [25]. Phosphinic acid porphyrins 5,10,15,20-tetrakis(4-methylphosphinatophenyl

Comparative biological effects of spherical noble metal nanoparticles (Rh, Pd, Ag, Pt, Au) with 4–8 nm diameter

• Alexander Rostek,
• Marina Breisch,
• Kevin Pappert,
• Kateryna Loza,
• Marc Heggen,
• Manfred Köller,
• Christina Sengstock and
• Matthias Epple

Beilstein J. Nanotechnol. 2018, 9, 2763–2774, doi:10.3762/bjnano.9.258

• passage, Lonza, Walkersville Inc., MD, USA) were cultured in cell culture medium RPMI1640 (GIBCO, Invitrogen GmbH, Karlsruhe, Germany) containing 10% fetal calf serum (FCS, GIBCO, Invitrogen GmbH) and L-glutamine (0.3 g L−1, GIBCO, Invitrogen GmbH) using 75 cm2 culture flasks (Falcon, Becton Dickinson

• by the addition of 0.2 mL cm−2 0.25% trypsin/0.05% ethylenediaminetetraacetic acid (EDTA, Sigma-Aldrich, Taufkirchen, Germany) for 5 min at 37 °C. Subsequently, the hMSC cells were collected and washed twice with RPMI1640/10% FCS. Subconfluent hMSC cells were seeded in 24-well cell culture plates

• (Falcon, Becton Dickinson GmbH, Heidelberg, Germany) at a density of 1.5 × 104 cells per well and incubated for 24 h at 37 °C under cell culture conditions. Nanoparticle solutions of 1.0, 0.5, 0.2, 0.1 and 0.05 g L−1 concentration were prepared in sterile, ultrapure water by serial dilution. To each

Size-selected Fe₃O₄–Au hybrid nanoparticles for improved magnetism-based theranostics

• Maria V. Efremova,
• Yulia A. Nalench,
• Eirini Myrovali,
• Anastasiia S. Garanina,
• Ivan S. Grebennikov,
• Polina K. Gifer,
• Maxim A. Abakumov,
• Marina Spasova,
• Makis Angelakeris,
• Alexander G. Savchenko,
• Michael Farle,
• Natalia L. Klyachko,
• Alexander G. Majouga and
• Ulf Wiedwald

Beilstein J. Nanotechnol. 2018, 9, 2684–2699, doi:10.3762/bjnano.9.251

• properties of 25 nm Fe3O4–Au hybrid NPs for in vitro MRI in 4T1 mouse breast adenocarcinoma cells have been recently demonstrated [84]. In summary, a r2 value of 276.9 mM−1·s−1 was obtained after 24 h of NP incubation with cell culture. Such an r2 value is suitable for MRI, although it was found to decrease

• as compared to the hybrids in water or agarose in line with previous cell culture experiments [85]. Here, we focus on the hyperthermia function of the hybrid materials in the same cell line. For this purpose, polymer-coated MNP-25 NPs were dispersed in RPMI medium at 3.6 mg·mL−1 Fe concentration

• case, 15 min and 30 min of exposure to AMF led to similar, yet improved, results: 100% cell death detected by apoptosis/necrosis activation in cell culture (Figure S6, Supporting Information File 1). Accordingly, no ROS activation is detected (Figure S7C and S7D, Supporting Information File 1) due to

Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe₂O₃ nanoparticles towards human tumor cells

• Zdeněk Plichta,
• Yulia Kozak,
• Rostyslav Panchuk,
• Viktoria Sokolova,
• Matthias Epple,
• Lesya Kobylinska,
• Pavla Jendelová and
• Daniel Horák

Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236

• cells (hMSCs), human cervix carcinoma cells of HeLa line and human osteosarcoma cells (MG-63) were obtained from cell culture collection of the University of Duisburg-Essen. MG-63 cells were cultured in DMEM medium, supplemented with 10% fetal calf serum (FCS), 100 U/mL penicillin, and 100 mg/mL

• grow for 24 h. The particles dispersed in another 100 µL of culture medium were added (Table 1) and the incubation continued for 24, 48, or 72 h. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; Sigma-Aldrich) was dissolved in PBS (5 mg/mL) and then diluted to 1 mg/mL in cell culture

Non-agglomerated silicon–organic nanoparticles and their nanocomplexes with oligonucleotides: synthesis and properties

• Asya S. Levina,
• Marina N. Repkova,
• Nadezhda V. Shikina,
• Zinfer R. Ismagilov,
• Svetlana A. Yashnik,
• Dmitrii V. Semenov,
• Yulia I. Savinovskaya,
• Natalia A. Mazurkova,
• Inna A. Pyshnaya and
• Valentina F. Zarytova

Beilstein J. Nanotechnol. 2018, 9, 2516–2525, doi:10.3762/bjnano.9.234

• complexes were predominantly detected in the cellular nuclei. The Si–NH2·ODN nanocomplexes demonstrated a high antisense activity against the influenza A virus in a cell culture at a concentration that was lower than their 50% toxic concentration by three orders of magnitude. Keywords: antiviral effect

• nanoparticles and nanocomplexes. We also demonstrated the biological activity of the latter with an example of inhibition of influenza A virus replication in cell culture. Results and Discussion Si–NH2 nanoparticles were synthesized by the hydrolysis of aminopropyltriethoxysilane (APTES). The resulting product

• influenza A virus (IAV) replication in the cell culture. The concentration of Si–NH2 and Si–NH2·ODN resulting in 50% MDCK cell death (TC50) was found to be 10–20 mM (for Si). The nontoxic concentration of the samples (0.014 mM for silicon) was used to study their ability to interact with the RNA target in

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• , in vitro antineoplastic potential. Further, in vitro cell culture experiments (such as cell morphology studies like AO/EB staining and Hoechst staining) support the fact that apoptosis is one of the possible mechanisms through which cancer cells die. FE-SEM images of CaP@5-FU NP-treated cells

• processed using NOVA RC1 software (version-1.0.26.922). Cell culture The in vitro study was carried out in human colon cancer (HCT-15), lung adenocarcinoma (A549) and mouse fibroblast (NIH 3T3) cell lines and were procured from the National Centre for Cell Science, Pune, India. Human colon cancer (Duke’s

• effect after a course of CaP@5-FU NPs, CaP NPs and free 5-FU treatment was determined by the mitochondrial dehydrogenase enzyme-mediated MTT assay. This included tests on preseeded HCT-15, A549 and NIH 3T3 cells at a density of 4 × 104 cells per well in a 96-well cell culture plate and followed

Biomimetic and biodegradable cellulose acetate scaffolds loaded with dexamethasone for bone implants

• Aikaterini-Rafailia Tsiapla,
• Varvara Karagkiozaki,
• Veroniki Bakola,
• Foteini Pappa,
• Panagiota Gkertsiou,
• Eleni Pavlidou and
• Stergios Logothetidis

Beilstein J. Nanotechnol. 2018, 9, 1986–1994, doi:10.3762/bjnano.9.189

• -Aldrich, Germany. N,N-Dimethylacetamide was obtained from Chem-Lab NV, Belgium. In MTT assay the cells used in this study were mice fibroblasts (L929) and phosphate-buffered saline (PBS), Dulbecco’s Modifies Eagle Medium (DMEM), 10% fetal bovine serum (FBS) and antibiotics were obtained from Gibco® Cell

• Culture. Preparation of polymer and drug solution The CA solution (20%, w/w) was prepared by dissolving CA in a mixture of acetone and dimethylacetamide and stirred with a magnetic stirrer over night at room temperature. The dexamethasone solution was created by adding dexamethasone to acetone and placed

Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants

• Reika Makita,
• Tsukasa Akasaka,
• Seiichi Tamagawa,
• Yasuhiro Yoshida,
• Saori Miyata,
• Hirofumi Miyaji and
• Tsutomu Sugaya

Beilstein J. Nanotechnol. 2018, 9, 1735–1754, doi:10.3762/bjnano.9.165

• . The stability of the different gelatin patterns could be controlled by the degree of genipin crosslinking. The gelatin patterns at 20 mM concentration of genipin and 41% crosslinking maintained a stable, patterned shape for at least 14 days in a cell culture medium. A cell morphology study showed that

• , we investigated the effect of genipin concentration on the stability of the different gelatin surface patterns in cell culture medium, as shown in Figure 2. The color of the crosslinked patterns gradually became a darker blue as the concentration of genipin increased from 1 to 20 mM. As shown in

• in the cell culture medium, gelatin surfaces with grooves (500 nm width and 500 nm height) were immersed in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS). After 1 h, 7 days, or 14 days of incubation, the immersed gelatin grooves were fixed with glutaraldehyde and

Perfusion double-channel micropipette probes for oxygen flux mapping with single-cell resolution

• Yang Gao,
• Bin Li,
• Riju Singhal,
• Adam Fontecchio,
• Ben Pelleg,
• Zulfiya Orynbayeva,
• Yury Gogotsi and
• Gary Friedman

Beilstein J. Nanotechnol. 2018, 9, 850–860, doi:10.3762/bjnano.9.79

• consumption rates of small individual cells. The general idea behind the proposed use of a double-channel pipette for oxygen consumption measurement by individual cells in a cell culture is illustrated in Figure 1a. The SEM images of two theta pipettes (whose cross-sections looks like the Greek letter θ

• individual cells in cell culture. Figure 5 illustrates that high resolution is achievable. In fact, it shows that the resolution is on the order of the pipette diameter and, since diameters smaller than micrometers are readily achievable [40][41][42], spatial resolution on the order of micrometers is

• selected in all studies to have the most accurate result with relative tolerance set to 10−6. a) Illustration of the double-barrel perfusion-based single-cell respirometry probe. The cell culture or tissue dish is shown on top of an x–y–z positioning set-up. The inset (1) shows tubing for the inlet and

Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumour cell growth inhibition

• Nagamalai Vasimalai,
• Vânia Vilas-Boas,
• Juan Gallo,
• María de Fátima Cerqueira,
• Mario Menéndez-Miranda,
• José Manuel Costa-Fernández,
• Lorena Diéguez,
• Begoña Espiña and
• María Teresa Fernández-Argüelles

Beilstein J. Nanotechnol. 2018, 9, 530–544, doi:10.3762/bjnano.9.51

• type with cell culture medium containing PrestoBlue (PB) reduced by untreated cells. As shown in Figure S4 (Supporting Information File 1), reading fluorescence emission at 590 nm before and after the addition of C-dots shows no interference of the C-dots on PB emission at 560 nm excitation wavelength

• set at 200 °C, and the collision cell energy between 17.5 and 52.5 eV. Cell culture HK-2 cells were grown in DMEM/F12 medium supplemented with 10% FBS, 100 U·mL−1 penicillin/100 μg·mL−1 streptomycin, 2.5 μg·mL−1 fungizone, and 5 μg·mL−1 human transferrin. LN-229 cells were maintained in DMEM high

• excitation wavelength of 560 nm. To assess the level of interference of the C-dots on the technique used to measure cell viability, untreated cells were similarly incubated with PB to obtain the reduced compound. The obtained cell culture medium containing the reduced compound was separated from the cells

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• ; mesenchymal stem cells; quantum dots; spheroids; 3D cell culture; Introduction The recent progress in the development of nanoscale agents opens up new perspectives for targeted drug delivery in cancer diagnostics, imaging and therapy. However, once administered into the body, nanoparticles (NPs) are rapidly

• uptake pathway in 3D conditions likely due to poor inhibitor penetrance into the spheroids. In general, 3D cultures are extensively studied given their potential to mimic cellular interactions and signal transduction occurring in in vivo conditions [14]. Briefly, 3D cell culture formation occurs on

• nanoparticle delivery vehicles to specifically target metastatic breast cancer cells. Experimental Cell culture Primary human skin mesenchymal stem cells (MSCs) from frozen primary cell stock were used in accordance with authorised approval from the Institute of Experimental and Clinical Medicine Ethics

Liquid-crystalline nanoarchitectures for tissue engineering

• Baeckkyoung Sung and
• Min-Ho Kim

Beilstein J. Nanotechnol. 2018, 9, 205–215, doi:10.3762/bjnano.9.22

• purposes, LCEs need to undergo additional physicochemical modifications. For example, Abbott and co-workers developed a cell-culture monitoring tool with which the orientational order of ECM-decorated nematic LCE substrates could be utilized for sensing ECM-attachment and proliferation of human embryonic

Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages

• Dimitri Vanhecke,
• Dagmar A. Kuhn,
• Dorleta Jimenez de Aberasturi,
• Sandor Balog,
• Ana Milosevic,
• Dominic Urban,
• Diana Peckys,
• Niels de Jonge,
• Wolfgang J. Parak,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2017, 8, 2396–2409, doi:10.3762/bjnano.8.239

• by analytical methods to assess their cellular uptake mechanisms and intracellular distribution after uptake at the single-cell level. Both NP types were provided to the cells both as single exposure, or combined as co-exposure. The NP behaviour in complete-serum-containing cell culture media was

• (ESEM), transmission electron microscopy (TEM), while quantification of gold and iron oxide was performed by inductively-coupled plasma optical emission spectrometry (ICP-OES). The endocytotic route was determined by the use of specific inhibitors. Results Particle characterisation in cell culture

• ). Depolarized dynamic light scattering measurements show that all particles analysed remained stable in both single and co-exposure experiments over 24 h in complete cell culture media (not shown). See Figures S1–S13 (Supporting Information File 1) for a full description of particle synthesis and

Evaluating the toxicity of TiO₂-based nanoparticles to Chinese hamster ovary cells and Escherichia coli: a complementary experimental and computational approach

• Alicja Mikolajczyk,
• Natalia Sizochenko,
• Ewa Mulkiewicz,
• Anna Malankowska,
• Michal Nischk,
• Przemyslaw Jurczak,
• Seishiro Hirano,
• Grzegorz Nowaczyk,
• Adriana Zaleska-Medynska,
• Jerzy Leszczynski,
• Agnieszka Gajewicz and
• Tomasz Puzyn

Beilstein J. Nanotechnol. 2017, 8, 2171–2180, doi:10.3762/bjnano.8.216

• , as described previously [53]. Toxicity to Chinese hamster ovary (CHO-K1) cells Nanoparticles were ground for 5 min using a mortar and pestle, suspended to a concentration of 1 mg/mL in complete cell culture medium with 0.1% pluronic F68 (cytotoxicity assay) or TSB (MIC determination) and sonicated in

• . Because Au nanoparticles absorb light in the visible region, the plates were centrifuged to avoid interference with the assay. At the next step, 100 µL of medium from each cell culture was transferred to a 96-well plate and the absorbance at 450 nm was measured. Cell viability was calculated as means of

Carbon nano-onions as fluorescent on/off modulated nanoprobes for diagnostics

• Stefania Lettieri,
• Marta d’Amora,
• Adalberto Camisasca,
• Alberto Diaspro and
• Silvia Giordani

Beilstein J. Nanotechnol. 2017, 8, 1878–1888, doi:10.3762/bjnano.8.188

• treated with only cell culture medium were used as a control. The cell viability percentage was above 80%, showing that CNOs exhibited moderate toxicity to the cells at the tested concentrations (Figure 6). The observed high viability of the HeLa cells treated with CNOs demonstrated their suitability for

• . Cell culture HeLa cells (obtained from a human cervix carcinoma) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum (FBS) (Life Technologies), 100 IU/mL penicillin and 100 μg mL−1 (Life Technologies) in humidified atmosphere at 37 °C

Synthesis and functionalization of NaGdF₄:Yb,Er@NaGdF₄ core–shell nanoparticles for possible application as multimodal contrast agents

• Dovile Baziulyte-Paulaviciene,
• Vitalijus Karabanovas,
• Marius Stasys,
• Greta Jarockyte,
• Vilius Poderys,
• Simas Sakirzanovas and
• Ricardas Rotomskis

Beilstein J. Nanotechnol. 2017, 8, 1815–1824, doi:10.3762/bjnano.8.183

• (Figure 3c), indicating that the Tween 80 was successfully coated onto the UCNPs. Additionally, dynamic light scattering (DLS) was employed to measure the hydrodynamic diameter of Tween-coated UCNPs in the cell culture medium as well as their surface zeta potential. The measured mean hydrodynamic diameter

Luminescent supramolecular hydrogels from a tripeptide and nitrogen-doped carbon nanodots

• Maria C. Cringoli,
• Slavko Kralj,
• Marina Kurbasic,
• Massimo Urban and
• Silvia Marchesan

Beilstein J. Nanotechnol. 2017, 8, 1553–1562, doi:10.3762/bjnano.8.157

• materials. Peptide self-assembled hydrogels are inherently biocompatible and biodegradable and thus are promising biomaterials for cell culture, regenerative medicine, tissue engineering, and drug delivery applications [22]. The identification of self-assembling peptides that are as short as possible is

Calcium fluoride based multifunctional nanoparticles for multimodal imaging

• Marion Straßer,
• Joachim H. X. Schrauth,
• Sofia Dembski,
• Daniel Haddad,
• Bernd Ahrens,
• Stefan Schweizer,
• Bastian Christ,
• Alevtina Cubukova,
• Marco Metzger,
• Heike Walles,
• Peter M. Jakob and
• Gerhard Sextl

Beilstein J. Nanotechnol. 2017, 8, 1484–1493, doi:10.3762/bjnano.8.148

• cytotoxicity of the NPs was tested by a cell culture based viability assay. Results and Discussion Synthesis and characterization of the multifunctional nanoparticles The synthesis of the CaF2:(Tb3+,Gd3+) NPs was carried out in analogy to the reported wet-chemical procedure that is based on a co-precipitation

• without appropriate surface modification have a disposition to agglomerate and sediment subsequently under physiological conditions because of their pH value and salt content [39][40][41]. One crucial requirement for the application of NPs in cell-culture experiments or animal testing is the stabilization

• in physiological media. In contrast to an electrostatically stabilization of the NPs, for example by capping the CaF2 NPs surface with citrate groups [28], we ensure the stability of the NPs in serum-containing cell-culture media in an electrosterical way. To this end, a polymer consisting of a

Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery

• Gamze Varan,
• Juan M. Benito,
• Carmen Ortiz Mellet and
• Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1457–1468, doi:10.3762/bjnano.8.145

• their drug encapsulation, release profile and anticancer activity on MCF-7 human breast cancer cell line in particular. Safety and apoptotic efficacy of blank and PCX-loaded cationic or anionic amphiphilic CD nanoparticles were evaluated with cell culture studies against a series of healthy and cancer

• therefore be suggested that the surface charge of nanoparticle is directly effective on the drug release profile. Cell culture studies In order to determine the safety of blank amphiphilic CD nanoparticles and the anticancer efficacy of PCX-loaded amphiphilic CD nanoparticles, L929 mouse fibroblast cells

• concentration dependent and that they are also non-hemolytic [24][45]. Therefore, these nanoparticles may be safe on healthy cells as drug carrying systems. To optimize the concentration of CD nanoparticles for cell culture studies, the inhibitory concentration 50 (IC50) value of PCX was calculated on MCF-7

Cationic PEGylated polycaprolactone nanoparticles carrying post-operation docetaxel for glioma treatment

• Cem Varan and
• Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1446–1456, doi:10.3762/bjnano.8.144

• Background: Brain tumors are the most common tumors among adolescents. Although some chemotherapeutics are known to be effective against brain tumors based on cell culture studies, the same effect is not observed in clinical trials. For this reason, the development of drug delivery systems is important to

• particles, and the drug release rate from the nanoparticles was slowed down to 48 h by dispersing the nanoparticles in a hydroxypropyl cellulose film. Cell culture studies revealed that docetaxel-loaded nanoparticles cause higher cytotoxicity compared to the free docetaxel solution in DMSO. Conclusion

• recurrence during the first 2 days. Cell culture studies Cytotoxicity assay for blank nanoparticles Mouse fibroblast cell lines L929 (recommended by the USP for the cytotoxicity evaluation of polymeric systems) were used to determine the cytotoxicity of blank nanoparticles with MTT assay. According to MTT

Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers

• Dong Ye,
• Mattia Bramini,
• Delyan R. Hristov,
• Sha Wan,
• Anna Salvati,
• Christoffer Åberg and
• Kenneth A. Dawson

Beilstein J. Nanotechnol. 2017, 8, 1396–1406, doi:10.3762/bjnano.8.141

• , Figure S1). Based upon previous experience, we limited exposure times to 6 hours in order to reduce the risk of released free dye and fragmentation of the nanoparticles, stemming from partial solubility in cell culture medium, which could confuse uptake and transport studies [42]. Nanoparticle size and

• injected before each measurement to calibrate the instrument, followed by 100 µL of the undiluted particle dispersion. Cell culture and exposure to nanoparticles Caco-2 epithelial cells (supplied by European Collection of Authenticated Cell Cultures) were cultured in complete Dulbecco's Modified Eagle