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Search for "isolation" in Full Text gives 940 result(s) in Beilstein Journal of Organic Chemistry. Showing first 200.
Discovery of antimicrobial peptides clostrisin and cellulosin from Clostridium: insights into their structures, co-localized biosynthetic gene clusters, and antibiotic activity
Beilstein J. Org. Chem. 2024, 20, 1800–1816, doi:10.3762/bjoc.20.159
- microbiota members. The habitat of C. cellulovurans, the strain of origin of the lanthipeptide clusters, appears to be plant-associated due to its metabolism and isolation environment (wood). Our results raise numerous questions regarding the ecological functions of the lantibiotics we discovered. Our
Oxidative fluorination with Selectfluor: A convenient procedure for preparing hypervalent iodine(V) fluorides
Beilstein J. Org. Chem. 2024, 20, 1785–1793, doi:10.3762/bjoc.20.157
- protocol using TCCA (4 equivalents) and KF (6 equivalents) was then applied to both bicyclic iodine(III) amides 11a and 11b, but these reactions also failed to form either difluoroiodane 7a or 7b. Following the successful preparation and isolation of difluoroiodane 6, we investigated its ability to
- increased by the stepwise incorporation of the trifluoromethyl groups into the sidearm, but trifluoroiodane 22 was less stable than bicyclic difluoro(aryl)-λ5-iodane 6. Conclusion In summary, we have developed a new strategy using Selectfluor for the convenient preparation and isolation of hypervalent
Ugi bisamides based on pyrrolyl-β-chlorovinylaldehyde and their unusual transformations
Beilstein J. Org. Chem. 2024, 20, 1773–1784, doi:10.3762/bjoc.20.156
- for two days and then heating the reaction mixture in a closed vessel at 80 °C for 3 hours (Scheme 5). This procedure led to the formation and isolation of the products 10d and 12e with yields which are similar to the tandem reaction. Using the example of bisamide 8c, it was found that changing the
Synthesis of polycyclic aromatic quinones by continuous flow electrochemical oxidation: anodic methoxylation of polycyclic aromatic phenols (PAPs)
Beilstein J. Org. Chem. 2024, 20, 1746–1757, doi:10.3762/bjoc.20.153
- multiple products were formed. The lability of the acetals prompted us to submit the crude intermediate directly to hydrolysis without prior isolation (Table 4). As mentioned above, having an aqueous reaction mixture in the electrochemical oxidation will give reduced yields. The overall yield of 9b from 6b
- of 1-chrysenol (3b) at different electron equivalents.a Anodic methoxylation of PAPs followed by hydrolysis in two separate steps.a Synthesis of o- and p-quinones without isolation of the acetal intermediate.a Electrochemical properties of PAPs. Supporting Information Supporting Information File 6
Chemo-enzymatic total synthesis: current approaches toward the integration of chemical and enzymatic transformations
Beilstein J. Org. Chem. 2024, 20, 1693–1712, doi:10.3762/bjoc.20.151
- reaction with biotin azide, which led to selective pull-down with streptavidin agarose and isolation of the probe–protein covalent complex. Proteomic analysis of the isolated proteins narrowed down the MaMO and MaDA candidates, including several berberine bridge enzyme (BBE)-like enzymes. This FAD-linked
- enzyme SfmCy2, lead to saframycin S (95) [100][101]. To convert the labile hemiaminal moiety at C21 to a more stable aminonitrile, the extracts of the microorganism containing 95 were treated with KCN, allowing the isolation as saframycin A (5) [89]. A notable feature of this biosynthetic machinery is
- 98 [104]. Without isolation of any intermediate from the simple synthetic substrates 88 and 96, rapid access to 98 was achieved in 13% yield over two pots based on peptidyl aldehyde 96. From chemo-enzymatically constructed scaffold 98, chemical manipulations of functional groups were investigated to
Polymer degrading marine Microbulbifer bacteria: an un(der)utilized source of chemical and biocatalytic novelty
Beilstein J. Org. Chem. 2024, 20, 1635–1651, doi:10.3762/bjoc.20.146
- not only a wide variety of polysaccharides including cellulose, agar, chitin, alginate, and xylan [8][9][10][11], but also plastics [4][12]. Progressing from their isolation and cultivation, natural product discovery from Microbulbifer bacteria is starting to gather steam [6][13][14]. The major
- -695 showed antimicrobial activity against E. coli [14]. Bioassay-guided fractionation led to the isolation of two new acetamidobenzoate esters: bulbiferate A (13) and bulbiferate B (14, Figure 6). Their structures were established on the basis of comprehensive spectroscopic and spectrometric data
- algae [17]. Further investigation indicated that the bioactive substance was mainly located in the cell-free supernatant of Microbulbifer sp. RZ01. Fractionation and spectroscopic characterization led to the isolation and identification of the extracellularly secreted molecule, 3,3´,5,5´-tetrabromo-2,2
Mining raw plant transcriptomic data for new cyclopeptide alkaloids
Beilstein J. Org. Chem. 2024, 20, 1548–1559, doi:10.3762/bjoc.20.138
- transcripts [37][38]. For our analysis, the previously distinct Chenopodioideae family was viewed as members of the Amaranthaceae family based on the most up to date Angiosperm Phylogeny Group classification (APG IV) [23]. Isolation of burpitides from C. americanus Three separate extractions were performed
Benzylic C(sp3)–H fluorination
Beilstein J. Org. Chem. 2024, 20, 1527–1547, doi:10.3762/bjoc.20.137
- predominately considered to be stable to isolation conditions, secondary and tertiary suffer from the elimination of HF, especially in the presence of silica gel or glass vessels. Therefore, benzyl fluorides have been derivatised, for example in C–O, C–N and C–C bond-forming reactions [18][19][20], thereby also
- several secondary and tertiary benzyl fluorides were unstable to silica during isolation or storage in glass vessels, and therefore, demonstrated several downstream diversifications of the benzyl fluorides. Sevov, Zhang and co-workers reported in 2023 a stable copper(III) fluoride complex that was capable
Synthesis of substituted triazole–pyrazole hybrids using triazenylpyrazole precursors
Beilstein J. Org. Chem. 2024, 20, 1396–1404, doi:10.3762/bjoc.20.121
- the triazole unit via a copper-catalyzed azide–alkyne cycloaddition. The developed methodology was used to synthesize a library of over fifty new multi-substituted pyrazole–triazole hybrids. We also demonstrate a one-pot strategy that renders the isolation of potentially hazardous azides obsolete. In
- 50 °C. This optimization allowed for the isolation of the corresponding azides 19a–v in yields of 51% to quantitative (Scheme 2), usually with durations of 3–16 h. Longer reaction times were necessary for some CN-substituted triazene derivatives (19o, 19q, 19s, 19v), especially in combination with
Synthetic applications of the Cannizzaro reaction
Beilstein J. Org. Chem. 2024, 20, 1376–1395, doi:10.3762/bjoc.20.120
- also simplifies product isolation and purification and improves the overall sustainability of the process [23][24]. However; specific reaction requirements ensure efficient and selective utilization of solvents. Marvi and Talakoubi carried out the Cannizzaro reaction [25] using montmorillonite K-10 and
Computation-guided scaffold exploration of 2E,6E-1,10-trans/cis-eunicellanes
Beilstein J. Org. Chem. 2024, 20, 1320–1326, doi:10.3762/bjoc.20.115
- epoxide 9, but the similar reaction with 2 yields gersemienol 8. Isolation yields are provided. (B and C) Results of DFT calculations on the protonation-induced cyclizations of 1 and 2. The energies of the cationic intermediates (italicized values) are not on the same energy scale as for the substrates
- be 3.5 kcal mol−1 higher in energy than the one shown, an energy difference that is larger than expected based on experiment. Cope rearrangement in trans-eunicellanes. (A) The 2E-trans-eunicellane undergoes thermal Cope rearrangement, yielding atropisomers 10a/10b; an isolation yield of 96% was
Sustainable tandem acylation/Diels–Alder reaction toward versatile tricyclic epoxyisoindole-7-carboxylic acids in renewable green solvents
Beilstein J. Org. Chem. 2024, 20, 1308–1319, doi:10.3762/bjoc.20.114
- bisepoxyisoindole-7-carboxylic acids were obtained in good yields after a practical isolation procedure. The results obtained in this study demonstrate the potential of vegetable oils and their renewable materials to provide a reaction medium that is more sustainable than conventional organic solvents in cascade
- as the reaction medium seven times under optimum conditions and the results for the cycloadduct 2k are shown in Figure 1. As shown in the figure, there is no significant decrease in product yield. The reaction conditions and isolation method used in the literature procedures for the previous
- transformations is the purification of the synthesized product, since these oils are non-volatile and non-polar. However, in this study, the choice of these materials as green solvents allows convenient isolation of the cycloadducts by precipitation followed by filtration with hexane or diethyl ether. Thus, IMDAF
Light on the sustainable preparation of aryl-cored dibromides
Beilstein J. Org. Chem. 2024, 20, 1076–1087, doi:10.3762/bjoc.20.95
- undetectable 1H NMR impurities. The application of the same protocol on o-xylene (1, Figure 3) cleanly gave dibromide 1a in almost quantitative yield. Some issues emerged during the isolation step, because of the high lacrimatory activity of 1a [53][54], while 3a lacks the same effect. Higher nucleophilic
A Diels–Alder probe for discovery of natural products containing furan moieties
Beilstein J. Org. Chem. 2024, 20, 1001–1010, doi:10.3762/bjoc.20.88
- agar plates (1 mg/L titer or 5 µM). However, this initial isolation was enabled by heterologous expression of their biosynthetic genes from Streptomyces coelicolor to produce higher titers of the MMF molecules compared to the native producer [7]. Given the challenges of isolating furans generally and
Enantioselective synthesis of β-aryl-γ-lactam derivatives via Heck–Matsuda desymmetrization of N-protected 2,5-dihydro-1H-pyrroles
Beilstein J. Org. Chem. 2024, 20, 940–949, doi:10.3762/bjoc.20.84
- presence of a methoxy group after the Heck–Matsuda reaction indicates methanolysis after arylation. Given the importance of the lactam rings, we envisioned a sequential Jones oxidation protocol without isolation of the methyl N,O-acetal products to obtain the corresponding lactams 4. As observed in
Direct synthesis of acyl fluorides from carboxylic acids using benzothiazolium reagents
Beilstein J. Org. Chem. 2024, 20, 921–930, doi:10.3762/bjoc.20.82
- unreliable isolation of acyl fluoride intermediates, we next considered whether BT-SCF3-mediated deoxyfluorination of carboxylic acids could be coupled with a subsequent acylation in an overall one-pot process. Selecting amines as nucleophilic coupling partners, a short optimisation study was carried out to
- the optimised one-pot conditions with benzylamine (4a) resulted in the formation of the desired amide product, however, significant by-products were also observed. Careful column chromatography of the crude reaction mixture allowed for the partial isolation and characterisation of the
- , complicating isolation (Scheme 3a). As Boc-deprotection is seemingly feasible under the reaction conditions, to avoid formation of by-product 6, the process was tested using the N-Cbz-valine (1s). Moreover, the BT-SCF3 reagent was substituted for the longer chain BT-reagent BT-SC5F11. The use of this
Three-component N-alkenylation of azoles with alkynes and iodine(III) electrophile: synthesis of multisubstituted N-vinylazoles
Beilstein J. Org. Chem. 2024, 20, 891–897, doi:10.3762/bjoc.20.79
- -membered aromatic azacycles, imidazole completely failed to undergo the present N-alkenylation, whereas the reaction of 1,2,4-triazole was too sluggish to allow for the isolation and unambiguous characterization of the expected product. Next, the reaction of pyrazole (2a) was explored using different
(Bio)isosteres of ortho- and meta-substituted benzenes
Beilstein J. Org. Chem. 2024, 20, 859–890, doi:10.3762/bjoc.20.78
- intramolecular crossed [2 + 2] cycloaddition strategy (Scheme 4C) [36]. They were able to avoid purification by column chromatography by transformation of BCH ester (±)-53 into BCH acid (±)-54, allowing isolation of pure 1,2-BCH (±)-54 by crystallisation. Through their synthesis they were able to access both
Activity assays of NnlA homologs suggest the natural product N-nitroglycine is degraded by diverse bacteria
Beilstein J. Org. Chem. 2024, 20, 830–840, doi:10.3762/bjoc.20.75
- they are found in bacteria found in widespread classes and environments. Herein, we report the characterization of five NnlA homologs. It is shown that all five homologs exhibited NNG degradation activity. Isolation and characterization of four of these homologs showed that all contain heme, the
Skeletal rearrangement of 6,8-dioxabicyclo[3.2.1]octan-4-ols promoted by thionyl chloride or Appel conditions
Beilstein J. Org. Chem. 2024, 20, 823–829, doi:10.3762/bjoc.20.74
- chiral auxiliary 10a, and following a survey of conditions and isolation protocols, a 90% yield was obtained when 10a was heated in the presence of 2 equivalents of SOCl2 and 5 equivalents of pyridine in DCE. Flash chromatography of the chloroalkyl ether 11a resulted in significant loss due to hydrolysis
- , although 11a was sufficiently stable for filtration through a pad of silica. Applying these conditions and isolation protocols to all 3,3-disubstituted alcohols 10c–f gave moderate to excellent yields of the rearrangement products 11c–f as single stereoisomers. The reactions of alcohols 10b,d,e also gave
- some sulfites 13b,d,e, attributed to the reduced steric hindrance in the chlorosulfite intermediate allowing for the second alcohol to approach prior to rearrangement. The isolation of these materials suggested that dialkyl sulfite formation could compete with the rearrangement if the neighbouring
Methodology for awakening the potential secondary metabolic capacity in actinomycetes
Beilstein J. Org. Chem. 2024, 20, 753–766, doi:10.3762/bjoc.20.69
- drug discovery. However, it is becoming difficult to obtain novel compounds because of repeated isolation around the world. Therefore, a new strategy for discovering novel secondary metabolites is needed. Many researchers believe that actinomycetes have as yet unanalyzed secondary metabolic activities
- inducing resistance, and this approach has contributed to the isolation of many new compounds [47]. The ribosome engineering method is based on the principle that a mutation in the ribosomal S12 protein, which is associated with the acquisition of antibiotic resistance, activates secondary metabolism. The
New variochelins from soil-isolated Variovorax sp. H002
Beilstein J. Org. Chem. 2024, 20, 692–700, doi:10.3762/bjoc.20.63
- iron cycle within the rhizosphere. This study focused on exploring the natural products of the soil-isolated Variovorax sp. H002, leading to the isolation of variochelins A–E (1–5), a series of lipohexapeptide siderophores. NMR and MS/MS analyses revealed that these siderophores share a common core
- , including soil-isolated plant pathogens. Results and Discussion Isolation and structure elucidation In this study, we explored the secondary metabolite potential of the soil-isolated Variovorax sp. H002, domesticated from the Medicinal Plant Garden of the Faculty of Pharmaceutical Sciences, Hokkaido
- , suggesting that Variovorax sp. H002 modulates the local rhizosphere microbiome by utilizing variochelins (Table 2). Conclusion In this study, we reported the isolation of new variochelin congeners 3–5, as well as the known variochelins 1 and 2, from soil-isolated Variovorax sp. H002. The lipopeptide
Regioselective quinazoline C2 modifications through the azide–tetrazole tautomeric equilibrium
Beilstein J. Org. Chem. 2024, 20, 675–683, doi:10.3762/bjoc.20.61
- limited the formation of diazide 13 and significantly elevated the yield of the desired product to 69% over two steps. When other sufinates were employed, the product failed to precipitate, necessitating isolation through preparative HPLC. Quantitative nuclear magnetic resonance (qNMR) yields were
Palladium-catalyzed three-component radical-polar crossover carboamination of 1,3-dienes or allenes with diazo esters and amines
Beilstein J. Org. Chem. 2024, 20, 661–671, doi:10.3762/bjoc.20.59
- temperature (rt), the desired unsaturated ε-AA derivative 4a was obtained in 75% isolated yield (Table 1, entry 1). Isolation and NMR analysis demonstrated that this model reaction provided amino acid 4a with good E-selectivity and excellent regioselectivity (E/Z = 91:9, 1,4-/1,2-addition >20:1). Control
Isolation and structure determination of a new analog of polycavernosides from marine Okeania sp. cyanobacterium
Beilstein J. Org. Chem. 2024, 20, 645–652, doi:10.3762/bjoc.20.57
- . Polycavernoside E (1) exhibited moderate antitrypanosomal activity against Trypanosoma brucei rhodesiense. Furthermore, the isolation of polycavernoside E (1) from marine cyanobacteria provides additional evidence that marine cyanobacteria, and not red algae, are responsible for the biosynthesis of
- hexane. The aqueous MeOH portion was purified by reversed-phase column chromatography (ODS silica gel, MeOH/H2O), automated flash chromatography (hexane/EtOAc), and repeated reversed-phase HPLC to give polycavernoside E (1, 0.5 mg as a colorless oil). The isolation of compound 1 was directed by its
- ) [7]. Isolation of polycavernoside E (1) In a manner analogous to [7], the collected cyanobacterium (340 g) was extracted with EtOH (0.5 L) for 10 days at room temperature (rt). The extract was filtered, and the residue was homogenized with a blender and re-extracted with EtOH (0.5 L) at room
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